DIY laboratory glove box

I belive during this time it is important to educate ourselves to understand how somthing exists in such a complex biosphere thats why i would like to share my Lab glove Box that i designed myself and i hope that there is somebody out there that would like to explore as well the microbiome.

The Box works for me so far, to cultivate different mushroom species
widout the additive Hepa Filter stream that i added

The Componets that you will need are listed below
and i will share the Fusion 360 file in case you want to change the size .

Print instructions

The Componets that you will need are listed below
and i will share the Fusion 360 file in case you want to change the size .

If you have a Laser Cutter i added the sizes in the model Files Folder if you want to change them use Fusion 360 (free) to resize "Great Tool"

Acryl-Glas 4 x 500 mm x 500mm x 3.6mm
Acryl-Glue (I would recommend to use the right glue )
Silicone gun (if you worked with clean cuts it should work without)

16 x M4 20mm (without HEPA - stream)
+16 x M4 20mm (HEPA -Stream)
°Zortrax HEPA and Carbon Filter
°Vacum Tubing 40mm
Window Sealing Tape (or you could print out a Sealing with TPU )

I Printet all Parts with ASA Filament from PureFil .
It worked for me put shouldn't be a problem to use PETG or even PLA.

Infill workt with 20 -30 %

To use the long parts cute them in 3 pieces in the PrusaSlicer

You will need suport for the Hepa Box

IMPORTANT : If you want to use the HEPA - Stream you will need some kind of Ventilation i used en old pump from a inflatable mattress and connectet input and output to the Hepa pices like this you will have littel to no problem with contamination from other spores and bakteria

I will update the post if there are any improvments
and i would be very happy to here from your experince

Happy Building !!!